Our confocal raman imaging technology leads the industry in speed, sensitivity and resolution. This document specifies the work instructions for the cmi witec confocal raman microscope located in the ground floor physiology lab, quadrangle building. It is easy to handle, needs less space to install than other instruments, data is accurate, after sales care is also good. If you see an area where more clarification is needed, if additional information is needed, or if you have suggestions on how to make this guide more useful in the lab, please contact. As scanning nearfield microscopy requires only minimal sample preparation if any, it is ideally suited to quickly and effortlessly image the optical properties of a sample with resolution below the diffraction limit. The alpha300 m is a highquality yet affordable micro raman system that provides the same outstanding performance and spectral resolution that other witec. Witecs alpha500 confocal raman microscopy for chemical. Confocal raman microscopy witec alpha 300r please refer to witec alpha300r confocal raman microscope user manual for the details of the operating procedure. Witecs solution for correlative ramansem imaging is now available for zeiss sigma 300, a field emission scanning electron microscope fe. All imaging techniques can be integrated within the same microscope system. The alpha300 r is a confocal raman microscopy system available in. Witec alpha 300r quick operation summary october 2018. Superior confocal raman imaging system witec quantum design.
The th confocal raman imaging symposium nov 15 2016. Confocal raman imaging with witec sensitivity resolution. The system has 2 lasers 532 nm and 785 nm and 2 spectrometers covering shifts in the visible and near infrared range. Abstract confocal raman microscopy enables 3d imaging of various samples solely based on chemical contrast. The alpha 300r plus confocal raman microscope from witec. It is capable of producing 3 dimensional optical and chemical information and features optical components that are all coupled by optical fibres. Witec is a leading german manufacturer of confocal and scanningprobe microscopes for stateoftheart raman, atomic force microscopy afm, scanning nearfield optical microscopy snom and developed the integrated raman imaging and scanning electron rise microscopy. Serial section raman tomography with 10 times higher depth.
Instructions for the alpha300 raman confocal microscope. Manual stage for highly accurate singlepoint spectrum acquisitions. Nearfield raman imaging is an exceptional microscopy technique which links chemical raman information to highresolution scanning nearfield optical microscopy snom. The confocal raman imaging system alpha300 r from witec offers the unique ability to acquire chemical information nondestructively with a resolution down to the optical diffraction limit 200 nm. While long recognized as the stateoftheart imaging system, ongoing development resulting from our innovative spirit keeps the witec s raman microscope alpha300 r at the forefront of the technology and sets the benchmark in terms of flexibility, sensitivity, speed and performance. Confocal raman imaging microscope witec alpha300 r raman microscope featureshigh speed, high resolution confocal raman imaging hyperspectral image generation excellent diffraction limited lateral resolution of 200 300 nm outstanding depth reso. One of the most informative and interactive symposia of recent years, said witec ceo dr joachim koenen about the th confocal raman imaging symposium held from 26th to the 28th september in ulm, germany. Confocal raman imaging witec alpha 300 coupled with atomic force microscope afm raman. Dec 21, 2010 the witec alpha 300r plus confocal raman microscope. Witec alpha 300r quick operation summaryoctober 2018. This user guide describes only operations that are specific to the structural and chemical analyser. Witec has launched the alpha300 ri inverted raman microscope. Confocal raman imaging with unprecedented performance in speed, sensitivity, and resolution hyper spectral image generation with the information of a complete raman spectrum at every image pixel excellent diffraction limited lateral resolution of 200 300 nm.
Raman spectroscopy and raman microscopy thermo fisher. It combines all the features of the alpha300 s and alpha300 r and many afm operation modes. Window should be adjusted see witec control manual section 3. Global analysis and opportunity evaluation 2016 2020.
Combined raman and scanning nearfield optical microscopy. The instrument is powered off the extension cords at the bottom. The video images acquired by the alpha300 alpha500 alpha700 system are. Ftir agilent spectrometer model 670ir combined with the microscope 620ir. Confocal raman imaging witec alpha 300 coupled with atomic force microscope. This manual may refer to other product names, which may be trademarked by their respective companies, and are used herein for identification purposes only. Our afm provides unrivaled versatility and user friendliness. The witec alpha 300r plus confocal raman microscope. For the user with challenging experimental requirements, the alpha300 rs facilitates confocal raman imaging in combination with scanning nearfield optical microscopy snom for optical imaging with resolution beyond the diffraction limit. The alpha 300r plus from witec is a confocal raman microscope with two scanning systems, a coarse motorized stage for large areas and a piezo stage for fine, sub nanometer adjustments. This allows to observe and analyze the distribution of different phases within a sample in ambient conditions without specialized sample preparation.
Witec s unique alpha300 ra microscope systems with raman and afm techniques combined. Raman, afm and snom allinone system witec quantum design. Witec is a leading manufacturer of confocal and scanningprobe microscopes for stateoftheart raman imaging, atomic force microscopy afm and scanning nearfield optical microscopy snom in addition to being the developer of the integrated raman imaging and scanning electron rise microscopy. The alpha300 access is a highquality yet affordable microraman system that provides the same outstanding. For information about all other features, the user is referred to the witec project manual. Witec alpha300 s scanning nearfield optical microscope camcor. Witec atomic force microscopes are developed and designed to allow combination with other imaging techniques such as confocal raman imaging. Aug 06, 2017 witecs solution for correlative ramansem imaging is now available for zeiss sigma 300, a field emission scanning electron microscope fesem. The witec alpha 300r plus confocal raman microscope youtube. This allows you to observe and analyze the distribution of different phases within a sample in ambient conditions without specialized sample preparation.
Confocal raman operation manual university of minnesota. The atomic force microscope witec alpha 300 ais a modular, user friendly atomic force microscope designed specifically for materials research, nanotechnology and life science. The witec raman is an advanced confocal raman microscope that combines fast. The alpha300 s is a user friendly scanning nearfield optical microscope snom that combines the advantages of snom, confocal microscopy and afm atomic force microscopy in a single instrument. Confocal raman microscopes from witec superior confocal raman imaging system. It combines all features of the alpha300 s and alpha300 r and many afm operation modes. It integrates a scientificgrade optical microscope for superior optical access, easy cantilever alignment and high resolution sample survey. Confocal raman microscopes from witec raman, afm and snom allinone system. The confocal raman imaging system alpha300 r offers the unique ability to acquire chemical information nondestructively with a resolution down to the optical diffraction limit 200 nm.
The witec alpha300 ra correlative microscope fuses the most powerful chemical and topographic imaging techniques into one fullyintegrated instrument. Hang the sign laser in user on the door when operating the instrument. Eyepiece color video camera motorized z stage system for automated approach led whitelight source for kohler illumination of tip and sample manual sample positioning in x and ydirection. Witecs alpha500 is the first instrument on the market to integrate atomic force microscopy afm and confocal raman imaging in a fully automated system incorporating a piezodriven scan stage and a motorized sample stage, the alpha500 enables raman imaging, highresolution surface topography imaging with afm on different sample positions, large area raman scans, and multipoint spectra. Witecs rise microscopy now available with zeiss sigma 300. The witec raman is an advanced confocal raman microscope that combines fast imaging, high spectral sensitivity and spatial resolution with excellent analysis software into a system designed around spectral imaging. Switching between the different modes can be easily done by rotating the objective turret. Typically, lateral resolutions of below 100 nm can be achieved.
From the raman spectra the distribution of two phases of cellulose green, blue and of hexane extratives red were imaged. This enables the user to take 3d raman images with submicron. Only these functionalities and their corresponding spectroscopy options are described inthismanual. The objective in position to start with should be 10x. You should hear a beeping sound right away, this is the microscope positioning itself. Cmi standard operating procedure confocal raman microscope. With the thermo scientific dxr3 family of raman instruments, you can use raman spectroscopy, microscopy, and imaging that quickly creates research grade images giving viewers instant information on the chemical, structural and elemental characteristics of their sample. User manuals, omax microscope operating guides and service manuals. Confocal raman afm images of traces of wood extractives on cellulose fibers. However, optical artifacts impair resolution and image quality in subsurface imaging. Turn on the laser 1 connect the power cord to the ion laser power supply. It combines the advantages of data acquisition from below with the established merits of 3d confocal raman imaging, a powerful and versatile technique that can chemically characterise samples nondestructively and without labeling or other specialised preparation.
Confocal raman microscopes from witec basic raman system. These unique characteristics have established the alpha300 r the preeminent confocal raman imaging system on the market. For the user with challenging experimental requirements, the alpha300 rs facilitates confocal raman imaging in combination with scanning nearfield optical microscopy for optical imaging with resolution beyond the diffraction limit. Online invia raman microscope user guide m98360797 sem operation manual provided by sem manufacturer. Witec confocal raman microscope alpha300r nanoscience. Raman imaging generates hyperspectral images with the information of a complete raman spectrum at every image pixel. The alpha300 r is witecs preeminent confocal raman imaging system, setting the benchmark in terms of flexibility, sensitivity, speed and performance. Witec alpha300r confocal raman microscope enables confocal raman imaging both surfacescan and depthscan with excellent resolution and capability of time series. The system has high confocality and boasts a minimum depth slice of 350 nm. Aug 24, 2017 witecs rise microscopy now available with zeiss sigma 300 scanning electron microscope read this article along with other careers information, tips and advice on biospace.
The witec raman is an advanced confocal raman microscope that combines fast imaging, high spectral sensitivity and spatial resolution with excellent analysis software into a system designed around imaging. Thus nearfield raman allows for the acquisition of complete highresolution confocal raman images. By simply rotating the microscope turret the user can then switch between the different methods. Witec offers manual, stepper motordriven and piezoactuated stages to. Please refer to witec alpha300r confocal raman microscope user manual for the details of the operating procedure. User manuals, zeiss microscope operating guides and service manuals.
22 717 448 412 25 419 564 775 1013 530 875 1502 1157 1210 1019 1149 979 362 10 344 1288 271 994 640 5 509 840 1315 34 389 1222 1214 580 4 163 121 1042 159 1224 163 1481 873 1055 173 146 708 448 1034 1332 1026